Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
C271V | Km for DL-DAP is 47fold higher compared to wild-type and kcat decreased by 1.5fold compared to wild-type.In contrast to wild-type C271V sDAPAL exhibits only 2fold stimulation in activity even when a high concentration of KCl is used | Salmonella enterica subsp. enterica serovar Typhimurium |
C299V | kcat and Km for DL-DAP are similar to wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
D125E | mutant does not show any activity with D-DAP at all | Salmonella enterica subsp. enterica serovar Typhimurium |
D125S | kcat value in mutant is reduced by 5.4fold for D-DAP compared to wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
D194P | mutant does not show any activity with either L-DAP or D-DAP. Kd for D-DAP shows a 5fold increase in mutant compared to wild-type. L-DAP does not bind at all to mutant D194P | Salmonella enterica subsp. enterica serovar Typhimurium |
D194S | mutant exhibits an 16fold decrease in kcat with L-DAP whereas activity with D-DAP is reduced only by factor 1.7 compared to wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
T385D | kcat value with DL-DAP as substrate is 69% of wild-type. Km value for D-Ser doubled in T385D mutant whereas the kcat value increased by 7fold | Salmonella enterica subsp. enterica serovar Typhimurium |
T385S | kcat value with DL-DAP as substrate is 86% of wild-type. Mutant exhibits a 2fold higher Kcat with D-Ser compared to wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant C271V sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.246 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.279 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, untagged sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.282 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.284 | - |
L-cysteine | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.293 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D194S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.3 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.3 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.33 | - |
D-serine | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.346 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.375 | - |
D-serine | pH 7.4, 30°C, mutant T385S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.378 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D194S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.382 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant T385S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.412 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125E sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.43 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant C299V sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.52 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.6 | - |
D-serine | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | addition of increasing concentrations (higher than 10 mM) enhances the activity by 10fold | Salmonella enterica subsp. enterica serovar Typhimurium | |
NaCl | addition of increasing concentrations enhances the activity by 2fold | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P66899 | - |
- |
Salmonella enterica | P40817 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | P40817 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2,3-diaminopropanoate + 2 H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + 2 NH3 | - |
? | |
D-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 | - |
? | |
DL-2,3-diaminopropanoate + 2 H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + 2 NH3 | - |
? | |
L-2,3-diaminopropanoate + 2 H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + 2 NH3 | - |
? | |
L-cysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAPAL | - |
Escherichia coli |
DAPAL | - |
Salmonella enterica |
diaminopropionate ammonia lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
diaminopropionate ammonia lyase | - |
Escherichia coli |
diaminopropionate ammonia lyase | - |
Salmonella enterica |
sDAPAL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
STM1002 | - |
Salmonella enterica |
ygeX | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
L-cysteine | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.67 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D194S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.17 | - |
D-serine | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
7.83 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
10.5 | - |
D-serine | pH 7.4, 30°C, mutant T385S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
20.83 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125E sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
26 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D194S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
34 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant C271V sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
34.17 | - |
D-serine | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
37.33 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, mutant D125S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
42 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant T385D sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
42.83 | - |
L-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
51.67 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant T385S sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
57 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, mutant C299V sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
60.17 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
69.17 | - |
DL-2,3-diaminopropanoate | pH 7.4, 30°C, untagged sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium | |
375.8 | - |
D-2,3-diaminopropanoate | pH 7.4, 30°C, sDAPL | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
pyridoxal 5'-phosphate | - |
Escherichia coli | |
pyridoxal 5'-phosphate | - |
Salmonella enterica |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | low expression of ygeX gene is shown by RT-PCR | down |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion shows loss of growth on DL-DAP, confirming that STM1002 is the locus encoding DAPAL. Inhibition by DL-DAP is rescued by transforming the strains with plasmids containing the STM1002 gene encoding DAPAL or supplementing the medium with casamino acids | Salmonella enterica |
malfunction | deletion shows loss of growth on DL-DAP, confirming that ygeX is the locus encoding DAPAL. Inhibition by DL-DAP is rescued by transforming the strains with plasmids containing the ygeX gene encoding DAPAL or supplementing the medium with casamino acids | Escherichia coli |